Tosefta for Nazir 4:1
דרבנין מאחר שנזיר טמא מביא אשם אינו נזיר:
If it becomes shadowy, the intermediate period (twilight) has arrived. If darkness has set in, so that the upper atmosphere has become indistinguishable from the lower, night has arrived. Rabbi says: When, at the period of the new moon, the sun commences to go down and the moon to appear, it is twilight.
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